PERISTALTIC PUMP APPLICATIONS

CHROMATOGRAPHIC TECHNIQUES

Compact and stackable LAMBDA peristaltic pumps are very well suitable for chromatography (together with LAMBDA OMNICOLL fraction collector and autosampler or as a stand alone instrument):

Liquid chromatography, LC
Taking samples
Automatic liquid handling
Collecting fractions / sampling
Gradient elution
Pouring gradient gels
Gradient formation
Column saturation with upward pump flow
Automatic gradient pump (with the help of PC software PNet)

LAMBDA Peristaltic pump with OMNICOLL used for chromatography applications

FERMENTATION AND CELL CULTURE RESEARCH

LAMBDA Peristaltic pumps are specially developed for long term continuous processes like in fermentations and cell cultures. With the sterilzable tubing, the peristaltic pump can be used for sterile and aseptic processes:

Feed pump and harvest pump (fed-batch culture, continuous culture, chemostat, turbidostat, perfusion)
pH control (addition of acid, base, buffers)
Antifoam control (addition of antifoam agent)
Sampling at particular time interval
Feed flow profile (exponential feed rate, automatic switch ON/OFF)
Aseptic sample addition/injection
Controlled addition of nutrients, minerals, trace elements
Media dispenser
Process optimization with C-feed in fed batch
Pump media with foot switch for filling agar plates
Follow growth kinetics
Quantify the metabolic activity of the culture

Utilization of LAMBDA laboratory Peristaltic pumps in fermentation and cell cultures

CHEMICAL & BIOCHEMICAL REACTIONS

Precise addition of liquids for titration
Visualisation of reaction velocity – hydrolysis of amides, esters, anhydrides, etc
Controlled addition of reactants during exothermic reactions (thermostat)
Acid or base addition to maintain pH (pH stat)
Control of oxidation and reduction potential
Measurement of enzyme activity of numerous enzymes (eg. esterases, acylases, lipases, proteases and others)

LAMBDA Peristaltic Pumps as pH stat, thermostat

SINGLE USE SYSTEM & PHARMACEUTICAL RESEARCH

Since it is a contamination sensitive process, LAMBDA peristaltic pumps can be used for precise addition or removal of the desired liquid
Sterile filtration
Dosing cell suspension
Process validation and drug trails